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1

Effect of Sugar Molecules on the Viscosity of High Concentration Monoclonal Antibody Solutions

Feng He, Christopher E. Woods, Jennifer R. Litowski, Lauren A. Roschen, Himanshu S. Gadgil, Vladimir I. Razinkov, Bruce A. Kerwin

Journal:

Pharmaceutical Research

Year:

2011

Language:

english

File:

PDF, 585 KB

english, 2011

2

The effect of sucrose hydrolysis on the stability of protein therapeutics during accelerated formulation studies

Douglas D. Banks, David M. Hambly, Joanna L. Scavezze, Christine C. Siska, Nicole L. Stackhouse, Himanshu S. Gadgil

Journal:

Journal of Pharmaceutical Sciences

Year:

2009

Language:

english

File:

PDF, 185 KB

english, 2009

3

A high-throughput UPLC method for the characterization of chemical modifications in monoclonal antibody molecules

Nicole Stackhouse, Amanda K. Miller, Himanshu S. Gadgil

Journal:

Journal of Pharmaceutical Sciences

Year:

2011

Language:

english

File:

PDF, 352 KB

english, 2011

4

LC/MS analysis of complex multiglycosylated human α1-acid glycoprotein as a model for developing identification and quantitation methods for intact glycopeptide analysis

Melanie M. Ivancic, Himanshu S. Gadgil, H. Brian Halsall, Michael J. Treuheit

Journal:

Analytical Biochemistry

Year:

2010

Language:

english

File:

PDF, 599 KB

english, 2010

5

Evaluation of effects of Fc domain high-mannose glycan on antibody stability

Yuefeng Lu, Kimberly Westland, Yu-heng Ma, Himanshu Gadgil

Journal:

Journal of Pharmaceutical Sciences

Year:

2012

Language:

english

File:

PDF, 906 KB

english, 2012

6

The LC/MS analysis of glycation of IgG molecules in sucrose containing formulations

Himanshu S. Gadgil, Pavel V. Bondarenko, Gary Pipes, Doug Rehder, Arnold McAuley, Natalie Perico, Tom Dillon, Margaret Ricci, Michael Treuheit

Journal:

Journal of Pharmaceutical Sciences

Year:

2007

Language:

english

File:

PDF, 487 KB

english, 2007

7

Comparative studies on discrete and concatemeric DNA-Sepharose columns for purification of transcription factors

Himanshu Gadgil, William L Taylor, Harry W Jarrett

Journal:

Journal of Chromatography A

Year:

2001

Language:

english

File:

PDF, 312 KB

english, 2001

8

Succinimide formation at Asn 55 in the complementarity determining region of a recombinant monoclonal antibody IgG1 heavy chain

Boxu Yan, Sean Steen, David Hambly, John Valliere-Douglass, Tim Vanden Bos, Scott Smallwood, Zac Yates, Thomas Arroll, Yihong Han, Himanshu Gadgil, Ramil F. Latypov, Alison Wallace, Aiching Lim, Gerd

Journal:

Journal of Pharmaceutical Sciences

Year:

2009

Language:

english

File:

PDF, 354 KB

english, 2009

9

Middle-down fragmentation for the identification and quantitation of site-specific methionine oxidation in an IgG1 molecule

Gary D. Pipes, Phillip Campbell, Pavel V. Bondarenko, Bruce A. Kerwin, Michael J. Treuheit, Himanshu S. Gadgil

Journal:

Journal of Pharmaceutical Sciences

Year:

2010

Language:

english

File:

PDF, 391 KB

english, 2010

10

Comparative studies on chemically and enzymatically coupled DNA–Sepharose columns for purification of a lac repressor chimeric fusion protein

F.Darlene Robinson, Himanshu Gadgil, Harry W. Jarrett

Journal:

Journal of Chromatography A

Year:

1999

Language:

english

File:

PDF, 597 KB

english, 1999

11

Reversed-phase liquid chromatography–mass spectrometry of site-specific chemical modifications in intact immunoglobulin molecules and their fragments

Da Ren, Gary Pipes, Gang Xiao, Gerd R. Kleemann, Pavel V. Bondarenko, Michael J. Treuheit, Himanshu S. Gadgil

Journal:

Journal of Chromatography A

Year:

2008

Language:

english

File:

PDF, 213 KB

english, 2008

12

Reversed-phase liquid chromatography of immunoglobulin G molecules and their fragments with the diphenyl column

Da Ren, Gary D. Pipes, David M. Hambly, Pavel V. Bondarenko, Michael J. Treuheit, David N. Brems, Himanshu S. Gadgil

Journal:

Journal of Chromatography A

Year:

2007

Language:

english

File:

PDF, 559 KB

english, 2007

13

Improving Mass Accuracy of High Performance Liquid Chromatography/Electrospray Ionization Time-of-Flight Mass Spectrometry of Intact Antibodies

Himanshu S. Gadgil, Gary D. Pipes, Thomas M. Dillon, Michael J. Treuheit, Pavel V. Bondarenko

Journal:

Journal of the American Society for Mass Spectrometry

Year:

2006

Language:

english

File:

PDF, 268 KB

english, 2006

14

Detection and Quantitation of IgG 1 Hinge Aspartate Isomerization: A Rapid Degradation in Stressed Stability Studies

Hambly, David M., Banks, Douglas D., Scavezze, Joanna L., Siska, Christine C., Gadgil, Himanshu S.

Journal:

Analytical Chemistry

Year:

2009

Language:

english

File:

PDF, 1.64 MB

english, 2009

15

Elucidation of two major aggregation pathways in an IgG2 antibody

Nicholas Van Buren, Douglas Rehder, Himanshu Gadgil, Masazumi Matsumura, Jaby Jacob

Journal:

Journal of Pharmaceutical Sciences

Year:

2009

Language:

english

File:

PDF, 565 KB

english, 2009

16

Proteome of monocytes primed with lipopolysaccharide: Analysis of the abundant proteins

Himanshu S. Gadgil, Karen M. Pabst, Francesco Giorgianni, Edward S. Umstot, Dominic M. Desiderio, Sarka Beranova-Giorgianni, Ivan C. Gerling, Michael J. Pabst

Journal:

Year:

2003

Language:

english

File:

PDF, 680 KB

english, 2003

17

DNA Affinity Chromatography of Transcription Factors

Himanshu Gadgil, Luis A. Jurado, Harry W. Jarrett

Journal:

Analytical Biochemistry

Year:

2001

Language:

english

File:

PDF, 292 KB

english, 2001

18

Top-down N-terminal sequencing of Immunoglobulin subunits with electrospray ionization time of flight mass spectrometry

Da Ren, Gary D. Pipes, David Hambly, Pavel V. Bondarenko, Michael J. Treuheit, Himanshu S. Gadgil

Journal:

Analytical Biochemistry

Year:

2009

Language:

english

File:

PDF, 6.07 MB

english, 2009

19

Paraffin-wax-coated plates as matrix-assisted laser desorption/ionization sample support for high-throughput identification of proteins by peptide mass fingerprinting

Nilesh S Tannu, Jian Wu, Vamshi K Rao, Himanshu S Gadgil, Michael J Pabst, Ivan C Gerling, Rajendra Raghow

Journal:

Analytical Biochemistry

Year:

2004

Language:

english

File:

PDF, 603 KB

english, 2004

20

Heparin elution of transcription factors from DNA-Sepharose columns

Himanshu Gadgil, Harry W Jarrett

Journal:

Journal of Chromatography A

Year:

1999

Language:

english

File:

PDF, 240 KB

english, 1999

21

Removal of cysteinylation from an unpaired sulfhydryl in the variable region of a recombinant monoclonal IgG1 antibody improves homogeneity, stability, and biological activity

Douglas D. Banks, Himanshu S. Gadgil, Gary D. Pipes, Pavel V. Bondarenko, Vipa Hobbs, Joanna L. Scavezze, Jun Kim, Xu-Rong Jiang, Venkat Mukku, Thomas M. Dillon

Journal:

Journal of Pharmaceutical Sciences

Year:

2008

Language:

english

File:

PDF, 348 KB

english, 2008

22

Affinity purification of DNA-binding proteins

Himanshu Gadgil, Shilpa A Oak, Harry W Jarrett

Journal:

Journal of Biochemical and Biophysical Methods

Year:

2001

Language:

english

File:

PDF, 125 KB

english, 2001

23

Comparison of LC and LC/MS methods for quantifyingN-glycosylation in recombinant IgGs

Sandipan Sinha, Gary Pipes, Elizabeth M. Topp, Pavel V. Bondarenko, Michael J. Treuheit, Himanshu S. Gadgil

Journal:

Journal of the American Society for Mass Spectrometry

Year:

2008

Language:

english

File:

PDF, 702 KB

english, 2008

24

Bi-column method for purification of transcription factors

Himanshu Gadgil, William L Taylor, Harry W Jarrett

Journal:

Journal of Chromatography A

Year:

2001

Language:

english

File:

PDF, 418 KB

english, 2001

25

Identification of cysteinylation of a free cysteine in the Fab region of a recombinant monoclonal IgG1 antibody using Lys-C limited proteolysis coupled with LC/MS analysis

Himanshu S. Gadgil, Pavel V. Bondarenko, Gary D. Pipes, Thomas M. Dillon, Douglas Banks, Jeffrey Abel, Gerd R. Kleemann, Michael J. Treuheit

Journal:

Analytical Biochemistry

Year:

2006

Language:

english

File:

PDF, 975 KB

english, 2006

26

Oligonucleotide trapping method for purification of transcription factors

Himanshu Gadgil, Harry W Jarrett

Journal:

Journal of Chromatography A

Year:

2002

Language:

english

File:

PDF, 642 KB

english, 2002

27

Screening and Sequencing of Glycated Proteins by Neutral Loss Scan LC/MS/MS Method

Gadgil, Himanshu S., Bondarenko, Pavel V., Treuheit, Michael J., Ren, Da

Journal:

Analytical Chemistry

Year:

2007

Language:

english

File:

PDF, 232 KB

english, 2007

28

Characterization of site-specific glycation during process development of a human therapeutic monoclonal antibody

Amanda K. Miller, David M. Hambly, Bruce A. Kerwin, Michael J. Treuheit, Himanshu S. Gadgil

Journal:

Journal of Pharmaceutical Sciences

Year:

2011

Language:

english

File:

PDF, 288 KB

english, 2011

29

DNA–support coupling for transcription factor purification: Comparison of aldehyde, cyanogen bromide and N-hydroxysuccinimide chemistries

Priya Sethu Chockalingam, Himanshu Gadgil, Harry W Jarrett

Journal:

Journal of Chromatography A

Year:

2002

Language:

english

File:

PDF, 632 KB

english, 2002

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